33 minute lecture on functional profiling of human cancer, using cell culture drug resistance testing on fresh human tumor specimens obtained by surgical biopsy. This lecture was given at Charite University Medical Center in Berlin, Germany on March 19, 2009 by Larry Weisenthal, MD PhD. The audience consisted primarily of oncologists and surgeons working in the field of gynecologic oncology. Thanks to Dr. Frank Kischkel, TherapySelect GmbH & Co. KG, Heidelberg, Germany
Posts Tagged ‘fresh human tumor culture’
Lecture on issues related to cell culture testing
Friday, November 13th, 2009Fresh human tumor cell culture atlas
Monday, October 12th, 2009My colleagues and I are working on an updated format for the Human Tumor Assay Journal, which now contains an atlas of fresh human tumors in short term suspension culture (click on Fresh Human Tumor Cell Culture Atlas illustrating examples of (1) the appearance of the fresh tumors immediately following scissor mincing and digestion with collagenase/DNAse, (2) appearance of “control” (cultured without drugs) tumor cells, following 96 hours of suspension culture, in anchorage-independent conditions, (3) appearance of cells when cultured with an “effective”/”active” drug, and (4) appearance of cells when cultured with an “ineffective”/”inactive” drug.
The atlas is a work in progress, but it now has sufficient numbers of examples of different classes of tumors that I hope it will be useful to workers in this field.
Comments and suggestions are welcome.
- Larry Weisenthal/Huntington Beach, CA mail@weisenthal.org